Analysis of ethanol in blood

     The Sida HS-16A headspace sampler(must connect to GC ) is an economical instrument configuration for measuring ethanol in blood. According to the national standard, drunk driving is defined as the concentration of ethanol in the blood of 80mg/100ml or more, while drinking and driving is defined as the concentration of enthanol in the blood of 20mg/100ml or more. To meet this quantitative requirement, the calibration curve for the method was generated in a wide range of 10mg/100ml to 160mg/100ml, so that both limits could be identified.

     The amount of alcohol-related traffic accidents and fatalities has steadily increased over the past decade. The government recently put a drunk driving law into effect. For law enforcement purposes, blood alcohol content is used to define intoxication, and provide a justifying measure of violation. As law enforcement agencies have risen for a reliable, fast, and accurate blood alcohol test. This application note provides a simpla, reliable, and fast solution that meets the latest national standards for measuring the ethanol content in blood.


1:GC Conditions 

Inlet settings 200℃, split ratio: 10:1

Column                         DB-ALC2 0.32 mm × 30m, 1.2μm

Column flow(N2)          12ml/min, constant flow

Oven temp program     40℃(7 min)

               Temperature:  250℃

FID setting           H2 Flow: 40ml/min

                           Air Flow: 400ml/min

                     Make up(N2): 45ml/min

2:HS Conditions


Oven             85℃

Loop              85℃

Transfer line 100℃


GC cycle time         15min

Press equib time    0.1min

Vial equib time       15min

Inject time             0.5 min